Fig. 2

The expression patterns of Mechanosensitive ion channel PIEZOs in human irreversible pulpitis. A The mRNA expression levels of PIEZO1 and PIEZO2 in normal pulp tissues (n = 21) and inflamed pulp tissues (n = 15) were examined using real-time PCR. B, C The gene expression levels of PIEZO1 and PIEZO2 in normal pulp tissues (n = 6) and inflamed pulp tissues (n = 6) in the public database, GSE77459, were analyzed. D The protein expression levels of PIEZO1 and PIEZO2 in normal pulp tissues (n = 5) and inflamed pulp tissues (n = 5) were examined using immunoblot. E The immunoblot results of protein expression levels of PIEZO1 and PIEZO2 were quantified. F Representative images of H&E staining and immunohistochemistry of the pulp are presented. Inflammatory cells (green arrows) and more blood vessels (blue arrows) are shown in inflamed pulp using H&E staining. Highly expressed PIEZO1 (yellow arrows) and highly expressed PIEZO2 (red arrows) are shown in immunohistochemistry images. P, pulp; D, dentin. * represents P < 0.05; ** represents P < 0.01; *** represents P < 0.001