Table 2 Polymerase chain reaction – sequence specific primers (PCR-SSP) for GSPP1 polymorphisms
Alleles | Sense primer | Antisense primer | Product size (bp) |
|---|---|---|---|
GSTP1 105Ile-114Ala (GSTP1_A) | 5´-ggACCTCCgCTgCAAATACA-3´ | 5´-CACATAgTCATCCTTgCCCg-3´ | 928 |
GSTP1 105Val-114Ala (GSTP1_B) | 5´-ggACCTCCgCTgCAAATACg-3´ | 5´-CACATAgTCATCCTTgCCCg-3´ | 928 |
GSTP1 105Val-114Val (GSTP1_C) | 5´-ggACCTCCgCTgCAAATACg-3´ | 5´-CACATAgTCATCCTTgCCCA-3´ | 928 |
GSTP1 105Ile-114Val (GSTP1_D) | 5´-ggACCTCCgCTgCAAATACA-3´ | 5´-CACATAgTCATCCTTgCCCA-3´ | 928 |
Primers for human growth hormone (internal control) | 5´-gCCTTCCCAACCATTCCCTT-3´ | 5´-TCACggATTTCTgTTgTgTTTC-3´ | 426 |