Fig. 3
From: CDH4 inhibits ferroptosis in oral squamous cell carcinoma cells
Knockdown of CDH4 suppresses the motility of OSCC. In the transwell migration assay and transwell invasion assay, the number of cells that passed through the filters was counted for HN30 cells A-B and SCC9 cells D-E. Representative images show the cells transfected with CDH4 that passed through the upper chamber of the transwell with and without (upper panel) Matrigel coating for HN30 cells C and SCC9 cells F. The numbers of migrating and invading cells were counted from at least five randomly selected fields of view under a microscope at 100X magnification. For the wound-heal assay, the representative images show the distances of cells passing through the scratch within a period of time for HN30 cells G and SCC9 cells I under a 40X microscope field of view. The mobility rate of cells was measured for HN30 cells H and SCC9 cells J. These experiments were repeated three times. (* indicates P < 0.05, **indicates P < 0.01, ***indicates P < 0.001, ****indicates P < 0.0001, Values are presented as means ± standard error.)