Fig. 2

Knockdown of CDH4 suppresses the proliferation and stemness of OSCC. The expression of CDH4 in four kinds of OSCC cells A-B. CDH4 expression was effectively reduced by siRNAs as measured by real-time PCR C and western blot D in HN30 cells. CDH4 expression was effectively reduced by siRNAs as measured by real-time PCR E and western blot F in SCC9 cells. After transfection with CDH4 siRNAs, the growth of HN30 cells G and SCC9 H cells was measured by CCK-8 assay. The colony formation assay showed that the proliferation of HN30 cells I and SCC9 cells J was decreased after CDH4 knockdown. The number of colonies was counted. The picture shows the cell sphere formation in different groups under a 40X microscope K, and the number of spheres was decreased in the siRNA-1 and siRNA-2 groups compared with the NC group L. These experiments were repeated three times. (* indicates P < 0.05, **indicates P < 0.01, ***indicates P < 0.001, ****indicates P < 0.0001, Values are presented as means ± standard error.)