Microbial identification from traumatized immature permanent teeth with periapical lesions using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Table 2 Isolated bacteria and their MALDI-TOF MS identification score

Pt	ID score of recovered bacteria
Pt	score ≥ 2	Score ≥ 1.7–1.9	score < 1.7–1.5	Score < 1.5
1	Slackia exigua Fusobacterium nucleatum Selenomonas infelix	Prevotella oralis
2	Strept sanguinis
3		Lactobacillus paracasei
4	Enterococcus faecalis	Staph aureus
5	Enterococcus faecalis Staph epidermidis	Streptococcus sanguinis
6	Staph warneri
7	Enterococcus faecalis Bacteroides ovatus	Prevotella negrescens
8			Clostsphenoides (Clostridium sp.)
9	Strept constellatus
10	Cupriavidus metallidurans Prevotella denticola Prevotella negrescens Strept anginosus
11	Strept pneumoniae Streptococcus mitis
12			Bacillus cereus (Bacillus sp.)
13	Enterococcus faecalis Strept anginosus Prevotella denticola Prevotella negrescens
14	Enterococcus faecalis Strept sanguinis			Mycob. genavense Bacillus atrophaeus Listeria grayi
15		Prevotella buccae	Acinetobacter calcoaceticus (Acinetobacter sp.) Staph epidermidis (Staphylococcus sp.)
16	Salmonella typhi Prevotella denticola Bacillus licheniformis Klebsiella pneumoniae
	27 (67.5%)	6 (15%)	4(10%)	3 (7.5%)

Note: *Eight isolates (20%) belonging to the Streptococcus genera were isolated from 7 teeth (43.75%)
In one tooth, Streptococcus pneumoniae&Streptococcus mitis were concomitantly isolated
^** Eight isolates (20% of total isolates) belonging to the Prevotella were detected in six teeth (37.5%) as P denticola&P negrescens were concomitantly detected in two teeth
^*** Five Enterococcus faecalis five isolates were isolated (12.5% of total isolates), from five teeth (31.25%)
^****The identification of four isolates with scores < 1.7—1.5 was accepted to the genus level only
^*****The three isolates (7.5%) with scores < 1.5 were considered unidentified

ISSN: 1472-6831