Fig. 4

Knockdown of EREG improved the osteo/dentinogenic differentiation ability of DPSCs under TNF-α stimulation. DPSCs were treated with 10 ng/mL TNF‐α. a Real-time RT-PCR results for the expression of EREG at 1, 2, 4 and 8 h after 10 ng/mL TNF‐α treatment of DPSCs. b ALP activity results of EREGsh and Scramsh DPSCs under TNF-α stimulation. c, d Alizarin red staining and calcium quantitative analysis results for EREGsh and Scramsh DPSCs under TNF-α stimulation. (E–G): Real-time RT-PCR results for the expression of DSPP, BSP and DMP-1 in EREGsh and Scramsh DPSCs under TNF-α stimulation. GAPDH was used as the internal control. Error bars represent the SD (n = 3). Student's t test were used to test statistical significance. *P ≤ 0.05. **P ≤ 0.01