Fig. 2

Immunofluorescence localization and expression of G-CSF in control group and mouse model of periodontitis. Images of the gingival junctional epithelium were taken by hematoxylin–eosin staining (A, B, E, F) with 20 × and 40 × objectives. The membranes of positive cells exhibited green fluorescence (C, G), while the nuclei exhibited blue fluorescence (D, H). Control group (A to D), periodontitis group (E to H), merged images (D, H). C, D, G and H: Bars, 20um. D, dentin; GE, gingival epithelium; CT, connective tissue; AB, alveolar bone