Table 2 Primer sequences of the investigated genes
Gene | Forward primer (5′- > 3′) | Reverse primer (5′- > 3′) | Annealing temperature (°C) | Product length (bp) |
|---|---|---|---|---|
α-SMA | TGGGGAAAGTAGATCGGACAG | AACAGTGGAAAGTTGGGGACA | 60 | 164 |
Vimentin | TTGTTCTCCAGGTACAGGTTACTA | GAGGACATCAACACCCAAATC | 60 | 89 |
GAPDH | GCGATACTCACTCTTCTACCTTCGA | TCGTACCAGGAAATGAGCTTGAC | 60 | 82 |