Skip to main content
Fig. 3 | BMC Oral Health

Fig. 3

From: The effect of chlorhexidine on dental calculus formation: an in vitro study

Fig. 3

Ca and Pi uptake inside biofilm masses exposed to CHG. Ca (a) and Pi (b) concentrations and Ca: Pi (c) molar ratio in biofilms were determined. The three-day-old biofilms were periodically exposed to 1 min applications of 0.12% CHG every 12 h and incubated for up to 2 days in BHI containing a calcifying solution. Distilled water was used as the control. Results are shown as the mean ± SD of four independent experiments. There was a significant difference among three experimental conditions (indicated by bracket, *p < 0.05). Incubation time affected calcium deposition (**p < 0.05). There was a significant difference in Ca: Pi molar ratio after 48 h incubation between the CHG treatment group and the others (***p < 0.05)

Back to article page