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Fig. 1 | BMC Oral Health

Fig. 1

From: Three/four-dimensional (3D/4D) microscopic imaging and processing in clinical dental research

Fig. 1

a Differential interference contrast (DIC) image displayed the anatomical structure of hard tissue dentin connected with soft pulp tissue from a tooth. b 2D images of a healthy control tooth showed scant expression of glial markers GFAP (green), S100B (red) and the overlay image with DIC channel to show the tooth orientation. c A matched set of 2D images showed increased expression of glial markers GFAP (green), S100B (red) and the overlay image with DIC channel for the orientation of an adult carious tooth. d Projection of 2D z-stack images of an adult carious tooth. e A 3D image generated by 2D z-stack data (d) from a thick carious specimen (78.89 μm)

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