Fig. 1

a Effect of LPS on the expression of SDF-1α and CXCR4 in HGF-1 cells. HGF-1 cells were incubated with different concentrations of LPS from P. gingivalis for 24 h. The cell lysates were then assayed to determine the expression of SDF-1α and CXCR4, Akt and NF-kβ p65, and the phosphorylation of Akt and NF-kβ p65 using Western blots. Membranes were stripped and re-probed with an anti-β-actin antibody as a loading control. Protein bands were quantified by densitometric analyses. The results are expressed as means ± S.D. (*p < 0.05). b Role of PI-3K/Akt in P. gingivalis LPS-stimulated SDF-1α expression. HGF-1 cells were pre-treated with or without LY294002 for 1 h and were then incubated with or without P. gingivalis LPS (500 μM) for 24 h. Cell lysates were assayed using Western blots to determine the expression of SDF-1α. Membranes were stripped and re-probed with an anti-β-actin antibody as a loading control. Protein bands were quantified by densitometric analyses. The results are expressed as means ± S.D. (*p < 0.05)