Figure 2From: Surface-associated MUC5B mucins promote protease activity in Lactobacillus fermentum biofilms Proteolytic activities of Lactobacillus fermentum biofilm populations in the presence or absence of MUC5B mucins. CSLM images showing biofilm cells growing in (a) the presence of a MUC5B environment or (b) a nutrient broth environment. Proteolytic activity was visualized by incubation with a FITC-conjugated casein substrate (green) for 1 hour. Cells were then counterstained using Syto 24 (red). The bar represents 20 μm and the inserts show four-fold enlargements of the main micrographs.Back to article page